How to Choose the Correct Pipette for your Laboratory?
A pipette is an indispensable tool in any laboratory, be it
chemistry, drug discovery, forensic, neuroscience, food, clinical, and many
other life science labs. Pipette simplifies liquid handling process and the appropriate
one will provide you with accurate results each time. However, there are
various factors that go into selecting the correct pipette.
If you are buying the pipette for the first time, then this
guide will help you narrow down your choices.
Types of Pipettes
There are a few basic types of pipettes like
the transfer pipettes, serological pipettes, mechanical, and digital pipettes.
Here we talk about the different types and what you might use them for.
Transfer pipettes
Transfer pipettes are disposable and designed
for the reliable and convenient transfer of liquid samples. As they are
disposable, thus, there is no chance of cross-contamination offering you
reliable results. Transfer pipettes are made of low-density polyethylene (LDPE)
and are available in extensive selection of capacities and various lengths to
accommodate different industry needs.
Serological pipettes
Serological pipettes are a clear choice for
accurate measurements. They are used in the laboratory to transfer milliliter
volumes of liquid, from less than 1 ml to up to 50 ml. These pipettes are made
of polystyrene (PS), designed with anti-drip tips, have aerosol barrier plug,
RNase and DNase free, and calibrated to deliver precise amount of sample. They
are available in various sizes including 1 ml pipette, 5 ml, 25 ml, and 50 ml.
Mechanical pipettes
Mechanical pipettes are valued for their
ergonomic design, ease of use, and reliability. Therefore, they are used for
various liquid handling tasks in research institutes, universities, pharma
labs, healthcare, and industrial laboratories. These pipettes feature
adjustable dispensing volume control, smooth plunger, tip ejector, and tip cone
that make frequent pipetting easy minimizing fatigue. Mechanical pipettes are
available as single and multi-channel form.
Digital pipettes
Digital or electronic pipettes offer fast,
precise, and comfortable pipetting. Their ergonomic design makes them very easy
to use. The pipettes have motorized piston with built-in error control to
improve precision and provide reliable results. It also has 3 speeds for
aspiration and pipetting and a high-quality, high-performance battery operation
allows rigorous use during busy times in the laboratories. A bright display
makes setting and reading easy, thus eliminating errors.
Once you know the pipette types, next thing
to look into is your application type.
What your application involves?
The crucial element of selecting the right
pipette is analyzing the application and the type of process. Depending on what
type of samples you will be working with, you have a couple of choices, air
displacement and positive displacement pipettes.
The air displacement pipette is driven by a
piston. When you push the plunger, the piston moves down allowing the air to
move out. When you release the plunger, a vacuum is created that draws the
desired volume of liquid into the tip. It is ideal for aqueous samples or standard
pipetting applications.
Positive displacement pipette is also driven
by a piston, but it is in direct contact with the liquid to be pipetted. The
tip in this pipette is a microsyringe composed of capillary and a piston that
displaces the liquid. The absence of air cushion makes positive displacement
pipette ideal for use with radioactive, corrosive, dense, and viscous samples
and used for DNA amplification and PCR techniques.
These are just a few of the lab pipettes
available for your needs. It is not always easy to figure out which equipment
is best but getting advice from a professional can help. Get in touch with the
experts at Microlit today and purchase the right pipette for your laboratory.
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